Peer-reviewed veterinary case report
Heartworm resistance to common drugs in dogs across the USA
By Curry, Emily et al.·Published in International journal for parasitology. Drugs and drug resistance·2025·Zoetis, United States·View original on PubMed →
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Original publication title: Surveillance of single nucleotide polymorphisms correlated to macrocyclic lactone resistance in Dirofilaria immitis from client-owned dogs across the United States.
- Species:
- dog
Plain-English summary
A study found that heartworm disease, caused by a parasite called Dirofilaria immitis, is becoming a concern for dogs across the United States due to some heartworms developing resistance to common preventive treatments. Blood samples from heartworm-positive dogs showed that about 31% of the samples contained heartworms that were resistant to these treatments. This resistance was found not only in the usual high-risk areas but also as far north as Michigan, suggesting that more dogs could be at risk than previously thought. Veterinarians are encouraged to stay informed about this issue and ensure that dog owners follow prevention guidelines closely.
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Abstract
Dirofilaria immitis is a parasitic filarial nematode and the causative agent of heartworm disease in canids and other species. Heartworm disease is predominantly managed via macrocyclic lactone (ML) - based chemoprophylactics. Through opportunistic sampling, genotypically and phenotypically confirmed ML-resistant D. immitis isolates have been isolated in the Lower Mississippi River Valley region (LMRV); however, the pervasiveness of resistant isolates in the USA has not been evaluated. This study aimed to evaluate the geographic distribution and prevalence of genotypically ML-resistant heartworms in client-owned dogs across the USA over a 3-year period. Owner consent was obtained to collect microfilaremic blood samples from heartworm-positive dogs from participating clinics. Veterinarians completed a questionnaire on the known history of each dog, including treatment and travel history. A total of 310 microfilaremic blood samples were collected from 45 geographically diverse veterinary clinics located in 22 states. Microfilariae were filtered from blood, DNA extracted utilizing the QIAGEN QIAamp DNA Micro Kit and samples sequenced by the Génome Québec Innovation Centre to determine allele frequencies at nine SNP sites previously correlated with ML resistance. The highly predictive 2-SNP model was used to identify genotypically susceptible, mixed, and resistant populations. Computational analysis indicated 111 (35.8 %) were genotypically susceptible, 96 (31.0 %) were genotypically resistant, and 103 (33.2 %) were genotypically mixed. The genotypically mixed and ML-resistant infections were located within and outside of the endemic LMRV, as far north as Michigan, which indicates canine populations outside of the LMRV are at increased risk for transmission of potentially ML-resistant heartworm infections than previously hypothesized. Veterinary practitioners across the USA need to be aware of the potential risks of ML resistance heartworm infections and ensure patient compliance with recommended prevention protocols.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/40779992/