Peer-reviewed veterinary case report
SYBR Green I-based real-time PCR targeting the rpoX gene for sensitive and rapid detection of Vibrio alginolyticus.
- Journal:
- Molecular and cellular probes
- Year:
- 2011
- Authors:
- Jing-jing, Zhao et al.
- Affiliation:
- The South China Sea Institute of Oceanology · China
Abstract
rpoX, a Vibrio alginolyticus specific stress regulating gene, was used to detect this fish pathogen by SYBR Green I-based real-time PCR. The specificity of the detection was confirmed in different samples. The minimum level of detection was 10(3) cells from pure culture and 10(2) cells from seawater.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21402144/