Synergistic effects of Chinese herbal formula combined with Microcin J25 againstandin calf diarrhea and clinical evaluation of preventive and therapeutic effects in the Ningxia region.

Abstract

INTRODUCTION: Calf diarrhea is one of the most common diseases causing significant economic losses in the livestock industry. This article aims to investigate the bacterial causes of calf diarrhea in the Ningxia region and to evaluate the synergistic antibacterial effect of Chinese Herbal Formula (CHF) and Microcin J25 (MccJ25) on pathogenic bacteria, as well as its clinical prevention and treatment effects on calf diarrhea. METHODS: A total of 100 diarrheic fecal samples were collected from calves across 10 cattle farms in the Ningxia region. Bacterial isolation and identification were performed to detectandstrains. Thesynergistic antibacterial effects of a Chinese Herbal Formula (CHF) combined with Microcin J25 (MccJ25) were evaluated using antimicrobial susceptibility testing. Furthermore, in vivo prevention and treatment trials were conducted to assess the efficacy of the CHF-MccJ25 combination against calf diarrhea, including evaluation of clinical symptoms, fecal microbial composition, and immune parameters. RESULTS: The results showed that out of 100 diarrheic calves fecal samples were collected from 10 cattle farms in the Ningxia Hui Autonomous Region. 97strains and 20strains were detected., the combination of Chinese Herbal Formula (CHF) and Microcin J25(MCJ) showed a synergistic inhibitory effect againstand. Moreover, the combined treatment also exhibited virulence-inhibitory activity., the combination effectively reduced the incidence of diarrhea in healthy calves in a dose-dependence. DISCUSSION: In summary, our study indicated that the combination of Chinese Herbal Formula (CHF) and Microcin J25(MCJ) effectively combated multidrug-resistantand, improving both prevention and treatment of calf diarrhea by enhancing immunity and restoring gut microbiota balance.