Peer-reviewed veterinary case report
TaqMan real-time PCR assay based on DNA polymerase gene for rapid detection of Orf infection.
- Journal:
- Journal of virological methods
- Year:
- 2011
- Authors:
- Bora, D P et al.
- Affiliation:
- Indian Veterinary Research Institute · India
Plain-English summary
Researchers developed a new test to quickly detect Orf virus infection in sheep and goats. This test, called real-time PCR (rt-PCR), can find very small amounts of the virus's DNA in samples from infected animals. It was shown to be very accurate, with a 100% success rate in identifying the virus and a 93.5% accuracy compared to another testing method. This means that the rt-PCR test could be a reliable option for diagnosing Orf in the field. Overall, the test worked well and could help veterinarians identify infections more quickly.
Abstract
Both conventional and real time PCR (rt-PCR) assays based on the amplification of a 103bp fragment from the DNA polymerase (DNA pol) gene (conserved, non-structural) of Orf virus (ORFV) were developed for detection and semi-quantitation of ORFV DNA from infected cell culture and clinical samples. The latter technique was based on TaqMan chemistry. The rt-PCR assay was specific and sensitive as it could detect as low as 3.5fg or 15 copies of ORFV genomic DNA. Both intra- (0.38-1.0%) and inter-assay (0.53-2.87%) variabilities of rt-PCR were within the acceptable range meaning the high efficiency and reproducibility of the assay. The rt-PCR was applied successfully to detect ORFV DNA from suspected clinical samples. Further, the assay has shown a relative diagnostic sensitivity and specificity of 100% and 93.5%, respectively, when compared to B2L gene based semi-nested PCR implying a wide potential of this rt-PCR for rapid field diagnosis of Orf in sheep and goats.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21946289/