Targeting MAPK10 with gga-miR-222b-5p suppresses apoptosis in DF-1 cells infected with the avian reticuloendotheliosis virus strain SNV(REV-SNV).

Abstract

Reticuloendotheliosis virus (REV), a retrovirus, causes reticuloendotheliosis in poultry, leading to severe immunosuppression. Evidence suggests that miR-222b-5p and mitogen-activated protein kinase 10 (MAPK10) exhibit tissue-specific differential expression patterns in the primary immune organs of specific pathogen-free chickens infected with the REV-SNV. In this study, we investigated the mechanisms underlying REV infection-induced changes in gga-miR-222b-5p and MAPK10 expression patterns. DF-1 cells were transfected with microRNA mimics or inhibitors to enable assessment of apoptosis, cell cycle progression, and cell viability, along with the differential expression patterns of gga-miR-222b-5p and MAPK10 in REV-infected cells. The results reveal that REV infection upregulated and downregulated miR-222b-5p and MAPK10, respectively, in a time- and dose-dependent manner. Overexpression of miR-222b-5p suppressed MAPK10 expression, thereby inhibiting apoptosis, enhancing cell viability, and accelerating cell cycle progression. These findings suggest that miR-222b-5p plays a vital role in REV infection-induced tumorigenesis.