Peer-reviewed veterinary case report
Early markers of osteoarthritis in dogs' joint fluid and blood
By H.B. Lee et al.·Published in Veterinární Medicína·2008·College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea, CZ·View original on DOAJ →
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Original publication title: Tartrate-resistant acid phosphatase, matrix metalloproteinase-2 and tissue inhibitor metalloproteinase-2 in early stages of canine osteoarthritis
- Species:
- dog
Plain-English summary
A group of beagle dogs with surgically induced knee injuries showed signs of early osteoarthritis (OA) when their joint fluid was tested. The researchers found that certain markers in the joint fluid, like tartrate-resistant acid phosphatase (TRAP) and matrix metalloproteinase-2 (MMP-2), were significantly higher in the dogs with OA compared to healthy dogs. Meanwhile, a tissue inhibitor (TIMP-2) was lower in the OA dogs. These findings suggest that measuring these markers in joint fluid could help veterinarians diagnose and monitor early OA in dogs.
People also search for: dog osteoarthritis symptoms · beagle joint pain treatment · early signs of arthritis in dogs
Abstract
The aim of this study was to determine if the activity of tartrate-resistant acid phosphatase (TRAP) in the synovial fluid (SF) and serum can be used as a marker for diagnosing the early stages of osteoarthritis (OA). We also wished to determine if identifiable differences in the concentrations of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) could be detected in SF between normal joints and OA joints for the diagnosis of early OA. Ten skeletally mature beagle dogs underwent a unilateral surgical transection of the cranial cruciate ligament and medial meniscectomy. Five sham-operated beagle dogs were used as controls. The synovial fluid was collected in 1, 2 and 3 months and examined by western blotting for MMP-2 and ELISA for TIMP-2. The activity of TRAP in the SF and serum was measured using a spectrophotometer. In addition, the presence of TRAP positive cells in the synovium was identified by enzyme histochemistry. The level of the activity of TRAP and MMP-2 in the SF from the induced OA dogs was significantly higher than that of the control over a three-month period (P < 0.05). The TIMP-2 level in the SF was significantly lower in the induced OA dogs than in the control. However, there was no difference in TRAP activity in the serum. Histochemistry revealed a higher number of TRAP positive cells in the synovium from the induced OA dogs. Based on these data, we conclude that the activity of TRAP, MMP-2 and TIMP-2 in SF can be used as a biomarker to diagnose and monitor the early stages of OA.
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Search related cases →Original publication on DOAJ: https://doi.org/10.17221/1921-VETMED