Taurine Attenuates-Induced Bovine Mammary Epithelial Cells Inflammation via Phosphoinositides/CaSignaling.

Abstract

Taurine may alleviate the inflammatory injury induced by() infection by regulating intracellular Calevels. However, the underlying mechanisms remain unclear. Infection leads to subversion of phosphoinositides (PIs) which are closely related to Casignaling. In order to investigate whether taurine regulates inflammation by means of PIs/ Casystems, competitive inhibitors of taurine (β-alanine) siTauT, siPAT1, siPLC, siCaN, siPKC, and inhibitors of PLC (U73122), PKC (RO31-8220), and CaN (FK 506) were used. The results indicate that taurine transfers the extracellular nutrient signal for intercellular innate immunity to phosphoinositides without a need to enter the cytoplasm while regulating intracellular Calevels during inflammation. Both the Ca-PKCα-NF-κB, and Ca-CaM-CaN-NFAT signaling pathways ofinfection and the regulatory roles of taurine follow activation of PIs/Casystems. These data increase our understanding on the mechanisms of multifunctional nutrient, taurine attenuated inflammatory responses caused byinfection, and provide theoretical support for the prevention of this disease.