Peer-reviewed veterinary case report
Techniques in electron microscopy of animal tissue.
- Journal:
- Veterinary pathology
- Year:
- 2014
- Authors:
- Cheville, N F & Stasko, J
- Affiliation:
- Department of Veterinary Pathology · United States
Plain-English summary
This research discusses how advancements in electron microscopy, a technique used to take very detailed pictures of animal tissues, are making it easier to analyze samples accurately. New software can improve the quality of these images by adjusting brightness, contrast, and color, and it can also help researchers select specific areas of the image for closer examination. However, preparing the samples can still lead to mistakes because the structures inside cells can change quickly when they are deprived of oxygen. The study offers guidelines to help ensure that the preparation and analysis of samples are done consistently across different types of electron microscopy. Overall, the improvements in technology and methods aim to enhance the reliability of results in this field.
Abstract
Technical improvements in electron microscopy, both instrumental and preparative, permit increasingly accurate analyses. Digital images for transmission electron microscopy (TEM) can be processed by software programs that automate tasks and create custom tools that allow for image enhancement for brightness, contrast and coloration; for creation of rectangular, ellipsoidal or irregular area selections; and for measurement of mean area and standard deviation. Sample preparation remains a source of error since organelles and spatial arrangements of macromolecules rapidly change after anoxia. Guidelines for maintaining consistency in preparation, examination and interpretation are presented for different electron microscopy (EM) modalities.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24114311/