Peer-reviewed veterinary case report
Changes in blood immune cells during dust mite allergy in Beagles
By Simpson, Andrew et al.·Published in The Journal of veterinary medical science·2009·Department of Small Animal Clinical Sciences, United States·View original on PubMed →
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Original publication title: Temporal dynamic changes of phenotypic expression of peripheral CD4 cells during environmental allergen challenge in an experimental model of canine atopic dermatitis: a pilot study.
- Species:
- dog
Plain-English summary
A group of Beagle dogs with atopic dermatitis (a skin allergy) were exposed to house dust mites to see how their immune cells reacted. The dogs developed itchy skin and lesions during the exposure, but after 17 days, their symptoms improved, and their immune cell counts returned to normal. This study suggests that monitoring specific immune cells could help in developing better treatments for dogs with skin allergies.
People also search for: dog skin allergy treatment · Beagle itching house dust mites · atopic dermatitis in dogs
Abstract
The immunopathology behind atopic dermatitis (AD) involves a myriad of inflammatory cells and their mediators. Thymus and activation-regulated chemokine (TARC) plays a significant role in the inflammatory phase by recruiting CCR4(+) T(H)2 cells. In addition, CD25(+) activated T cells further propagate the allergic response after sensitization by producing cytokines. The purpose of this pilot study was to evaluate how exposure to a common allergen (house dust mite, HDM) would affect the proportions of circulating CD4(+)CCR4(+) T(H)2 cells and CD4(+)CD25(+)activated T cells in an experimental model of AD using high-IgE Beagles. In this experimental model, previously sensitized Beagles develop lesions and pruritus upon allergen challenge consisting of 3-day environmental exposure, 3 hours/day. Blood samples were obtained before, during, and after the end of challenge (days 0, 2, 4, and 17). Clinical signs were evaluated and scored at the same time points. Peripheral blood mononuclear cells (PBMCs) were isolated and used for flow cytometry to identify proportions of CD4(+)cells positive for either CCR4 or CD25 receptors. Both CD4(+)cell types (CD25(+) and CCR4(+)) peaked at day 17, when clinical signs had resolved. It is proposed that the increase of circulating CD4(+)CCR4(+) and CD4(+)CD25(+)cells most likely demonstrates the sensitization status of atopic individuals after environmental allergen challenge. Understanding of these two cell types could prompt additional research concerning therapeutic drugs for AD.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/19801897/