The ameliorative role of-loaded chitosan nanoparticles oninduced acute lung injury: Targeting TLR/NF-κB signaling pathways.

Abstract

BACKGROUND: Acute lung injury (ALI) is a severe condition distinguished by inflammation and impaired gas exchange in the lungs.a common bacterium, can cause ALI through its virulence factors.is a medicinal plant that has been traditionally used to treat a variety of illnesses due to its anti-inflammatory properties. Chitosan nanoparticles are biocompatible and totally biodegradable materials that have shown potential in drug delivery systems. AIM: To explore the antibacterial activity of-loaded chitosan nanoparticles (AV-CS-NPs) againstandwith advanced techniques. METHODS: The antibacterial efficacy of AV-CS-NPs was evaluated through a broth microdilution assay. In addition, the impact of AV-CS-NPs oninduced ALI in rats was examined by analyzing the expression of genes linked to inflammation, oxidative stress, and apoptosis. Furthermore, rat lung tissue was scanned histologically. The rats were divided into three groups: control, ALI, and treatment with AV-CS-NPs. RESULTS: The AV-CS-NPs that were prepared exhibited clustered semispherical and spherical forms, having an average particle size of approximately 60 nm. These nanoparticles displayed a diverse structure with an uneven distribution of particle sizes. The maximum entrapment efficiency of 95.5% ± 1.25% was achieved. The obtained findings revealed that The minimum inhibitory concentration and minimum bactericidal concentration values were determined to be 5 and 10 ug/ml, respectively, indicating the potent bactericidal effect of the NPs. Also,infected rats explored upregulation in the mRNA expression ofandcompared to healthy control groups. AV-CS-NP treatment reverses the case where there was repression in mRNA expression ofandcompared to-treated rats. CONCLUSION: These NPs can serve as potential candidates for the development of alternative antimicrobial agents.