Peer-reviewed veterinary case report
How RT-PCR helps diagnose canine distemper in dogs
By I Nyoman Suartha et al.·Published in Jurnal Veteriner·2008
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Original publication title: THE APLICATION OF REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF CANINE DISTEMPER
- Species:
- dog
Plain-English summary
Fifteen out of twenty dogs showing symptoms of canine distemper were tested using a special lab technique called reverse transcriptase-polymerase chain reaction (RT-PCR) to confirm the presence of the virus. Canine distemper can cause serious illness, including fever, coughing, and neurological issues. The RT-PCR method was able to detect the virus in the nasal swabs of these dogs, confirming its effectiveness for diagnosing this disease. Early diagnosis is crucial for treatment and management of affected dogs.
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Abstract
<span style="color: #29363d; font-family: Tahoma, Arial, Helvetica, sans-serif; font-size: 14.666666984558105px; font-style: italic; text-align: justify; background-color: #ddeaf4;">A study was conducted to apply reverse transcriptase-polymerase chain reaction (RT-PCR) technique for the confirmative diagnosis of canine distemper in dogs. Twenty mongreal dogs with clinical symptoms of canine distemper were used in this study. The viral RNA was isolated from nasal swab using Trizol® and transcribed into cDNA using random primers 5’ACAGGATTGCTGAGGACCTAT 3’. The cDNA was amplified in one step RT-PCR using primers 5’-ACAGGATTGCTGAGGACCTAT-3’ (forward) and 5’- CAAGATAACCATGTACGGTGC-3’ (backward). A single band of 300 bp which was specific for canine distemper virus CDV) was detected in fifteen out of twenty samples. It is therefore evident that confirmative diagnostics of canine distemper disease can be established with RT-PCR technique.</span><div><span style="color: #29363d; font-family: Tahoma, Arial, Helvetica, sans-serif; font-size: 14.666666984558105px; font-style: italic; text-align: justify; background-color: #ddeaf4;"><br /></span></div>
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