The Bacteriophage vB_CbrM_HP1 Protects Crucian Carp AgainstInfection.

Abstract

is an opportunistic pathogen that induces aquatic infections in fish and turtles. In this study, a bacteriophage that infects, named vB_CbrM_HP1, was isolated from sewage. This phage belongs tofamily,subfamily, Mooglevirus genus. We also used the phage to treat crucian carp infection caused byfor the first time. vB_CbrM_HP1 was relatively stable at temperatures ranging from 4 to 60&#xb0;C and pH values ranging from 3 to 11 but float slightly. When the multiplicities of infection (MOI) was 0.0001, the titer reached a maximum of 4.20 &#xd7; 10PFU/ml. As revealed from the results of whole genomic sequence analysis, the total length of vB_CbrM_HP1 was 89335 bp, encoding 135 ORFs, 9 of which were <75% similar to the known sequences in NCBI. The phage vB_CbrM_HP1 showed a highly efficient bactericidal effect againstbothand., vB_CbrM_HP1 was capable of effectively killing bacteria (the colony count decreased by 4.7 log units at 5 h)., administration of vB_CbrM_HP1 (1 &#xd7; 10PFU) effectively protected crucian carp against fatal infection caused by. Phage treatment reduced the levels of inflammatory factors. All these results demonstrated the potential of vB_CbrM_HP1 as an alternative treatment strategy for infections caused by.