Peer-reviewed veterinary case report
The CSFV DNAChip: a novel diagnostic assay for classical swine fever virus.
- Journal:
- Journal of virological methods
- Year:
- 2014
- Authors:
- Kim, Yong Kwan et al.
- Affiliation:
- Viral Disease Division · South Korea
Plain-English summary
Researchers have created a new test called the CSFV DNAChip to quickly and accurately identify different types of the classical swine fever virus (CSFV) in pigs. They took samples from pigs and used a special process to extract the virus's genetic material, which was then tested with the DNAChip. This new method was able to correctly identify the virus in 91 out of 157 samples, matching results from more traditional testing methods. The CSFV DNAChip is expected to be a valuable tool in efforts to eliminate this virus in pig populations, as it provides fast and reliable results.
Abstract
A novel assay, the CSFV DNAChip, was developed to clearly and rapidly discriminate three genotypes of classical swine fever virus (CSFV). Total RNA was extracted from clinical samples and then subjected to a one-step reverse-transcription polymerase chain reaction (RT-PCR) using Cy3-labeled primers from the 5' non-coding region (NCR) of CSFV. Amplicons were hybridized to the CSFV DNAChip and fluorescence scanning was performed for detection of CSFV. A cut-off fluorescence intensity value of 5000 was determined by two-graph receiver operating curve (TG-ROC) analysis. The limit of detection values for the developed DNA chip assay were 0.313ng/μL for amplicon concentration and 1TCID50/100μL for virus titer. Using the developed DNA chip, 157 field samples (91 CSFV-positive and 66 CSFV-negative) were investigated. The genotypes determined by the CSFV DNAChip agreed completely with those determined by nucleotide sequence analysis of the viral genome. The developed CSFV DNAChip will be helpful in implementing a CSFV eradication strategy, as it provides a rapid and accurate diagnostic assay that can discriminate easily among CSFV genotypes.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24698761/