Peer-reviewed veterinary case report
Detecting and telling apart dog respiratory infections
By Wang, Lih-Chiann et al.·Published in Journal of virological methods·2017·School of Veterinary Medicine·View original on PubMed →
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Original publication title: The detection and differentiation of canine respiratory pathogens using oligonucleotide microarrays.
- Species:
- dog
Plain-English summary
A group of dogs with respiratory issues were tested for various viruses and bacteria that can cause similar symptoms, like coughing and sneezing. Researchers developed a new testing method using a microarray chip that could detect multiple pathogens at once, including canine distemper virus and Bordetella bronchiseptica. This new method was found to be more effective than traditional testing, identifying infections in a higher percentage of samples. The microarray could help veterinarians diagnose and treat respiratory diseases in dogs more accurately.
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Abstract
Canine respiratory diseases are commonly seen in dogs along with co-infections with multiple respiratory pathogens, including viruses and bacteria. Virus infections in even vaccinated dogs were also reported. The clinical signs caused by different respiratory etiological agents are similar, which makes differential diagnosis imperative. An oligonucleotide microarray system was developed in this study. The wild type and vaccine strains of canine distemper virus (CDV), influenza virus, canine herpesvirus (CHV), Bordetella bronchiseptica and Mycoplasma cynos were detected and differentiated simultaneously on a microarray chip. The detection limit is 10, 10, 100, 50 and 50 copy numbers for CDV, influenza virus, CHV, B. bronchiseptica and M. cynos, respectively. The clinical test results of nasal swab samples showed that the microarray had remarkably better efficacy than the multiplex PCR-agarose gel method. The positive detection rate of microarray and agarose gel was 59.0% (n=33) and 41.1% (n=23) among the 56 samples, respectively. CDV vaccine strain and pathogen co-infections were further demonstrated by the microarray but not by the multiplex PCR-agarose gel. The oligonucleotide microarray provides a highly efficient diagnosis alternative that could be applied to clinical usage, greatly assisting in disease therapy and control.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/28189583/