The Effects of Aflatoxin Bon Liver Cholestasis and Its Nutritional Regulation in Ducks.

Abstract

The aim of this study was to investigate the effects of aflatoxin B(AFB) on cholestasis in duck liver and its nutritional regulation. Three hundred sixty 1-day-old ducks were randomly divided into six groups and fed for 4 weeks. The control group was fed a basic diet, while the experimental group diet contained 90 &#x3bc;g/kg of AFB. Cholestyramine, atorvastatin calcium, taurine, and emodin were added to the diets of four experimental groups. The results show that in the AFBgroup, the growth properties, total bile acid (TBA) serum levels and total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), and glutathione (GSH) liver levels decreased, while the malondialdehyde (MDA) and TBA liver levels increased (< 0.05). Moreover, AFBcaused cholestasis. Cholestyramine, atorvastatin calcium, taurine, and emodin could reduce the TBA serum and liver levels (< 0.05), alleviating the symptoms of cholestasis. The qPCR results show that AFBupregulated() and() gene expression and downregulated() gene expression in the liver, and taurine and emodin downregulatedandgene expression (< 0.05). In summary, AFBnegatively affects health and alters the expression of genes related to liver bile acid metabolism, leading to cholestasis. Cholestyramine, atorvastatin calcium, taurine, and emodin can alleviate AFB-induced cholestasis.