Peer-reviewed veterinary case report
Stem cell artery treatment helps blood flow in dog hip bone death
By Jin, Hongting et al.·Published in International orthopaedics·2012·Zhejiang Chinese Medical University, China·View original on PubMed →
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Original publication title: The effects of autologous bone marrow mesenchymal stem cell arterial perfusion on vascular repair and angiogenesis in osteonecrosis of the femoral head in dogs.
- Species:
- dog
Plain-English summary
A group of healthy male Beagle dogs with osteonecrosis of the femoral head (a condition where the bone in the hip loses blood supply) received a treatment involving their own bone marrow stem cells to see if it would help repair blood vessels in the affected area. After four to eight weeks, the dogs that received the stem cell treatment showed improved blood flow and better blood vessel growth compared to those that only received saline. The results suggest that this treatment could enhance healing in the femoral head by improving its blood supply.
People also search for: Beagle hip pain treatment · dog osteonecrosis stem cell therapy · improving blood supply in dogs
Abstract
PURPOSE: The purpose of this study was to observe the effects of marrow mesenchymal stem cell (MSCs) arterial perfusion on vascular repair and angiogenesis in osteonecrosis of the femoral head (ONFH). METHODS: Twelve healthy male adult Beagle dogs were randomly divided into two groups: group A (the control group) and group B (the MSCs arterial perfusion group). ONFH animal models were established by hip dislocation and liquid nitrogen. At the same time, MSCs were obtained, cultured and proliferated. After three weeks, arterial perfusion was performed in all animals. Group B was given 1 ml MSCs (5 × 10(6)-1 × 10(7)/ml), while 0.9 % normal saline was used in group A. After four weeks or eight weeks, the dogs were put to death. The changes of main arteries, the expression of vascular endothelial growth factor (VEGF), VEGF mRNA and microvessel density (MVD) of ONFH were observed. All the data were analysed by SPSS13.0. RESULTS: In digital subtraction angiography (DSA), after four or eight weeks of treatment, the quantity and diameter of the main arteries of the femoral head in group B were improved, compared to group A (P < 0.05,P < 0.01). Concerning histology and immunohistochemistry, after four or eight weeks of treatment, the expression of VEGF and MVD were significantly higher than that of group A (P < 0.05, P < 0.01). For real-time quantitative polymerase chain reaction (RT-PCR), after four or eight weeks of treatment, the expression of VEGF mRNA in group B was significantly higher than that of group A (P < 0.05, P < 0.01), and after eight weeks of treatment, the expression of VEGF mRNA were significantly higher than that of four-weeks treatment in group A (P <0.01). CONCLUSIONS: MSCs arterial perfusion can promote vascular repair and angiogenesis and then improve blood supply and repair of femoral head.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/23064553/