The Guangsi Yulin Decoction Inhibits Oxidative Stress and Inflammation-Induced Spermatogenesis Dysfunctionthe///Pathway.

Abstract

Guangsi Yulin Decoction (GSYLD) is a traditional Chinese medicine (TCM) formula with therapeutic efficacy against spermatogenesis dysfunction. However, the underlying mechanism of the alleviation remains unclear. To investigate the effects of GSYLD onpolyglycolide (GTW)-induced spermatogenesis dysfunction in mice and to explore associated mechanisms, particularly its role in oxidative stress and inflammation. A total of 60 (7-8-week-old) BALB/c mice were grouped into control (NC), model (GTW), low-dose GSYLD (GSYLD-L, 0.27 g/kg/d), high-dose GSYLD (GSYLD-H, 1.08 g/kg/d), and PTEN inhibitor (1.08 g/kg/d GSYLD-H + 0.3 mg/kg PTEN inhibitor). After eight weeks, the model was validated, and semen quality was evaluated by assessing sperm vitality and concentration. Testicular tissues were analyzed by H&E staining, while Western blotting and RT-qPCR (Reverse Transcription Quantitative PCR) were used to assess the therapeutic efficacy of the GSYLD. In comparison to the GTW group, the GSYLD-H group showed a significant improvement in sperm vitality and concentration (< .01). GSYLD upregulated,, and, while downregulatingmRNA expression (< .001). A parallel recovery was observed at the protein level. Specifically, the GSYLD-H group exhibited a significant amelioration of oxidative stress and inflammation, marked by reduced MDA, TNF-&#x3b1;, and IL-6 levels (< .01) and enhanced SOD activity (< .001) relative to the GTW group. GSYLD alleviated GTW-induced spermatogenesis dysfunction, potentially through///-mediated regulation of oxidative stress and inflammation.