Peer-reviewed veterinary case report
Genipin kills bacteria causing infected corneal ulcers in dogs in lab
By Slenter, Inge J M et al.·Published in Investigative ophthalmology & visual science·2026·Department of Clinical Sciences, Netherlands·View original on PubMed →
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Original publication title: The In Vitro Antibacterial Effects of Genipin Against Pathogens Commonly Associated With Infected Ulcerative Keratitis: A Canine Preliminary Study.
- Species:
- dog
Plain-English summary
A study tested a substance called genipin for its ability to fight bacteria that can cause eye infections in dogs, particularly those with corneal ulcers. The researchers found that genipin was effective against several types of bacteria, including some that are resistant to common antibiotics. However, it did not show any antibacterial effects against one specific bacteria, Pseudomonas aeruginosa, within the time frame of the tests. Overall, genipin showed promise as a potential treatment for these infections, especially at higher concentrations. More research is needed to fully understand how well it works in real-life situations.
People also search for: dog eye infection treatment · corneal ulcer in dogs · genipin for dog eye problems
Abstract
PURPOSE: To evaluate genipin as a novel antimicrobial therapeutic agent with established crosslinking abilities by investigating its antibacterial activity against canine ocular pathogens and exploring its pharmacodynamic profile. METHODS: Nineteen bacterial strains were selected from frozen stock, including ten Staphylococcus pseudintermedius (five of which were methicillin-resistant [MRSP]), five Pseudomonas aeruginosa, and four Streptococcus canis. All strains were isolated from dogs with corneal stromal ulcerations. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were examined; subsequently, time-kill assays were performed. Viability counts were performed at 0, 10, 30, 60, and 120 minutes. RESULTS: Blue discoloration of the contents in the wells impeded the determination of the MIC values for all pathogens. MBC values ranged from 0.1% for MRSP, 0.075%-0.125% for S. pseudintermedius and P. aeruginosa, and 0.25%-0.5% for S. canis. Time-kill analysis demonstrated a mean bacteriostatic effect of genipin against staphylococci at 1 × MBC after 120 minutes, and a mean bactericidal effect after 120 minutes at 2.5 × MBC and after 60 minutes at 5 × MBC. No antibacterial effect was measured for P. aeruginosa within the tested timeframe and genipin concentrations. CONCLUSIONS: Genipin exhibited promising antibacterial activity against all tested isolates, including MRSP. We suggest that genipin acts as a concentration-dependent antimicrobial agent, demonstrating bactericidal activity at higher concentrations against staphylococci. P. aeruginosa appeared less susceptible to the short-term effects of genipin. Further investigation is warranted to elucidate genipin's full antimicrobial potential and clinical performance.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/41800841/