The interaction between free Cain host cells and invasion of E. tenella.

Abstract

Eimeria tenella is the most pathogenic and common coccidia that causes chicken coccidiosis. The intracellular free Caof the host cell is closely related to the invasion, development, and proliferation of intracellular parasites. To determine the dynamic changes of intracellular free Caand its function in the process of E. tenella invading host cells, we established a chick embryo cecal epithelial cells model of E. tenella infection. Chick embryo cecal epithelial cells were treated with different Casignal inhibitor, respectively, and then infected with E. tenella. The results showed that extracellular Ca, Cachannels on the cell membrane, IP3R ion channels on the endoplasmic reticulum membrane, and RyR ion channels regulated the free Cain cecal epithelial cells. Through fluorescence labeling and invasion rate detection, we found that the intracellular Cadid not change significantly during the invasion of E. tenella, but its stability was critical to the invasion of parasites.