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Peer-reviewed veterinary case report

The K/BxN arthritis model.

Journal:
Current protocols in immunology
Year:
2008
Authors:
Monach, Paul A et al.
Affiliation:
Harvard Medical School · United States
Species:
rodent

Abstract

Mice expressing both the T cell receptor (TCR) transgene KRN and the MHC class II molecule A(g7) (K/BxN mice) develop severe inflammatory arthritis, and serum from these mice causes a similar arthritis in a wide range of mouse strains, due to autoantibodies recognizing glucose-6-phosphate isomerase (GPI). K/BxN transgenic mice have been useful for investigating the development of autoimmunity, and the serum transfer model has been particularly valuable in eliciting mechanisms by which anti-GPI autoantibodies induce joint-specific inflammation. This unit describes detailed methods for the maintenance of a K/BxN colony, induction of arthritis by serum transfer, clinical evaluation of arthritis, and measurement of anti-GPI antibodies.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/18491295/