Peer-reviewed veterinary case report
Rapid DNA test to diagnose Babesia canis infection in dogs
By Adaszek, Ł et al.·Published in Polish journal of veterinary sciences·2013·Department of Epizootiology and Infectious Diseases·View original on PubMed →
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Original publication title: The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs.
- Species:
- dog
Plain-English summary
A group of dogs suspected of having Babesia canis canis infections, which can cause symptoms like fever and lethargy, were tested using a new DNA-based method called LAMP (loop-mediated isothermal amplification). This test was able to quickly and accurately detect the infection in all samples tested, even at very low levels of the parasite's DNA. The results indicate that LAMP could be a reliable option for veterinarians to diagnose this serious disease in dogs.
People also search for: dog Babesia canis symptoms · rapid test for dog infections · how to diagnose Babesia in dogs
Abstract
The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that this method has high specificity and sensitivity and can be applied in analytical laboratories in diagnosis of canine babesiosis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/23691588/