The preventive effect of chlorogenic acid on cisplatin-induced acute kidney injury in mice.

Abstract

Acute kidney injury (AKI) is a common clinical syndrome. Chlorogenic acid (CGA) is a natural polyphenol with antioxidant and anti-inflammatory properties. In this study, 60 male Kunming mice were randomly assigned to 6 groups: Control (CON), Cisplatin (CIS), CGA, CIS + CGA, CIS + furosemide (FUR), and FUR. Kidney injury markers, inflammatory indicators, antioxidant enzyme activities, oxidative products, antioxidant proteins, and kidney morphology were assessed using ELISA, histology, and Western blot. Preventive CGA supplementation significantly reduced levels of creatinine (Cr), BUN, KIM-1, and MDA, while restoring the enzymatic activities of SOD, GSH-Px, CAT, and T-AOC. CGA also increased the expression of Nrf2 and GCLC proteins and decreased the expression of Keap1 protein. Levels of IL-1β, IL-2, and IL-6 were reduced, while IL-10 levels were elevated. These results indicate that preventive CGA supplementation effectively mitigates CIS-induced AKI by enhancing antioxidant capacity, attenuating inflammatory responses, and ameliorating kidney structural damage.