Peer-reviewed veterinary case report
The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae.
- Journal:
- Folia microbiologica
- Year:
- 2008
- Authors:
- Lobová, D et al.
- Affiliation:
- Institute of Microbiology and Immunology
Abstract
Single-chain antibodies (scFv) specific to Brachyspira hyodysenteriae were isolated from a phagemid library. Recombinant Bhlp 29.7 protein was used for scFv selection and individual clones were tested by ELISA and immunofluorescent test; four unique clones were isolated. One of selected clones was able to bind specifically B. hyodysenteriae in ELISA and immunofluorescence test. This is the first report of species-specific recombinant antibodies against B. hyodysenteriae.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/19381477/