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Peer-reviewed veterinary case report

The use of MPB70 and MPB83 to distinguish between bovine tuberculosis and paratuberculosis.

Journal:
Comparative immunology, microbiology and infectious diseases
Year:
2010
Authors:
Marassi, Carla D et al.
Affiliation:
Laboratory of Veterinary Bacteriology · Brazil

Abstract

In order to demonstrate the potential to distinguish paratuberculosis (PTB) from bovine tuberculosis infection (TB), ELISAs with M. bovis-specific MPB70 or MPB83 as capture antigens were developed and tested on two groups of cattle: Group A comprised 23 animals positive for Mycobacterium avium paratuberculosis (Map) and TB free. Group B comprised 48 animals from a Map free herd during the previous 5 years, but confirmed as tuberculous by positive results on PPD testing and M. bovis culture. Results demonstrated a significant difference (p<0.01) between reactivity of sera from these groups, encouraging the study of purified proteins to differentiate between both diseases.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/19735941/