Peer-reviewed veterinary case report
Platelet growth factor cells speed skin wound healing in dogs
By Kim, Namyul et al.·Published in Histology and histopathology·2020·Research Institute for Veterinary Science, South Korea·View original on PubMed →
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Original publication title: Therapeutic effects of platelet derived growth factor overexpressed-mesenchymal stromal cells and sheets in canine skin wound healing model.
- Species:
- dog
Plain-English summary
A group of dogs with skin wounds were treated with special cells derived from fat (called Ad-MSCs) that had been enhanced with a growth factor known as platelet-derived growth factor (PDGF). This treatment was compared to regular Ad-MSCs without the growth factor. After 5 and 10 days, the dogs that received the PDGF-enhanced cells showed faster healing and better skin regeneration than those that received the regular cells. The PDGF-enhanced cell sheets were particularly effective at promoting healing by creating more granulation tissue, which is important for wound recovery. Overall, using PDGF-enhanced cells or sheets can significantly help dogs heal from skin wounds.
People also search for: dog skin wound treatment · PDGF for dog wounds · healing skin wounds in dogs
Abstract
Adipose-derived mesenchymal stromal cells (Ad-MSCs) have excellent potential for skin wound repair. Moreover, platelet-derived growth factor (PDGF) has strong wound healing properties. The purpose of the present study was to compare the healing effects of PDGF-overexpressing canine allogeneic Ad-MSCs (PDGF-MSCs) and their cell sheets (PDGF-CSs) as compared to unexpressed Ad-MSCs (U-MSCs) and their cell sheets (UCSs) in a cutaneous wound healing model induced upon dogs. In in vitro study, the expression of immunomodulatory and growth factors was assessed by qRT-PCR. In in vivo study, cells and sheets were transplanted into a square-shaped full-thickness (1.5×1.5 cm) skin defect model created in 12 dogs. After 5 and 10 days, wounds were harvested and evaluated macroscopically and histopathologically. The qRT-PCR results showed that the PDGF-B gene was significantly upregulated (p<0.05) in PDGF-CS and PDGF-MSCs groups. Upon gross analysis of the wound, all stromal cells and their sheet groups showed accelerated (p<0.05) cutaneous wound healing compared to the negative control groups. As compared to U-MSCs and UCSs, the PDGF-MSCs showed significant epithelization on days 5 and 10 of healing, whereas PDGF-CSs showed improved epithelization only on day 10. In the granulation tissue analysis, PDGF-CSs and UCSs promoted more formation (p<0.05) of upper granulation tissue, collagen, and activated fibroblasts than PDGF-MSCs, and U-MSCs. Especially, the PDGF-CSs presented the highest formation and maturation of granulation tissue among all groups. All considered, PDGF overexpressed stromal cells or cells sheets can improve cutaneous wound healing in a canine model.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31876285/