Therapeutic efficacy of LysGH15 against necrotising pneumonia caused byin a rabbit model.

Abstract

INTRODUCTION: () is one of the most important zoonotic pathogens and can be transmitted to humans through the meat diet routes, causing necrotising pneumonia. METHODS: This study investigated the therapeutic effect of bacteriophage lysin LysGH15 on necrotising pneumonia in rabbit model caused by. RESULTS: In theexperiments, 50 μg/mL LysGH15 not only significantly reduced the viable count (approximately 3.24 × 10 CFU/g) of chicken meat stored at 4°C for 48 h but also effectively reduced the viable count of chicken meat thawed at 4°C and 30°C, with reductions of approximately 1.42 × 10 CFU/g and 2.78 × 10 CFU/g, respectively. In theexperiments, a single intranasal administration of 300 μg/rabbit increased the survival rate of rabbits to 60%. At 72 h postinfection, the number of bacteria in the lung tissues of the rabbits treated with LysGH15 was 7 × 10 CFU/g, which was significantly lower than that in the lung tissues of rabbits treated with PBS (7.76 × 10 CFU/g) or linezolid (6.38 × 10 CFU/g). In addition, LysGH15 treatment alleviated lung tissue damage in infected rabbits and significantly reduced the levels of Panton-Valentine leukocidin (PVL), alpha-toxin (Hla), and the cytokines IFN-, TNF-, and IL-8 in their lung tissues, similar to those in rabbits treated with linezolid. DISCUSSION: These results suggest that LysGH15 has the potential to be used as a novel antimicrobial agent for the treatment of necrotising pneumonia caused by.