Topical Vancomycin is More Efficient than Povidone-Iodine Treatment in Controlling Bacterial Growth in Methicillin-ResistantKeratitis Model in Rabbits.

Abstract

To evaluate the efficacy of topical vancomycin and povidone iodine (PI) application on methicillin-resistant(MRSA) keratitis model in rabbits. MRSA keratitis was induced by injecting 0.1&#x2009;mL MRSA containing 1000 colony-forming units (CFU) into central cornea of right eyes of 24 New Zealand White rabbits. Animals were divided into four groups (&#x2009;=&#x2009;6): control (treated with balanced salt solution), 50&#x2009;mg/mL topical vancomycin, 5% topical PI, and combination; examined before and after treatment, and corneal tissues were harvested for analysis at 9th hour of treatment. Bacterial load was determined as: 7.63&#x2009;&#xb1;&#x2009;0.82 log10 CFU/g in control group, 6.95&#x2009;&#xb1;&#x2009;1.66 log10 CFU/g in PI group, 4.67&#x2009;&#xb1;&#x2009;0.77 log10 CFU/g in combination group, and 4.33&#x2009;&#xb1;&#x2009;0.71 log10 CFU/g in vancomycin group (&#x2009;=&#x2009;0.001). Median of total clinical score increased significantly from 7 [range: 5-8] to 11.5 [range: 11-15] (&#x2009;=&#x2009;0.001) in control group, did not change (6 [range: 5-8] to 7 [range: 5-7];&#x2009;=&#x2009;0.695) in vancomycin group, increased significantly from 7 [range: 5-8] to 12.5 [range: 10-14] (&#x2009;<&#x2009;0.001) in PI group, increased significantly from 6.5 [range: 5-7] to 8 [range: 7-9] in combination group (&#x2009;=&#x2009;0.002). Post-treatment clinical scores for chemosis, conjunctival injection, iritis, hypopyon, epithelial erosion, and corneal infiltrate were significantly lower in vancomycin-treated groups compared to others (&#x2009;<&#x2009;0.05). In PI-treated groups, especially scores for chemosis, conjunctival injection, epithelial erosion and corneal infiltrate were significantly higher than vancomycin (&#x2009;<&#x2009;0.05). Topical vancomycin significantly inhibited bacterial growth in MRSA keratitis. However, PI was ineffective in controlling this growth; additionally, exerted toxic effect on ocular surface. When vancomycin was combined with PI, no additional increase in efficacy of treatment was detected compared to only vancomycin.