Peer-reviewed veterinary case report
Testing inactivated erysipelas vaccines without mice?
By Balks, E et al.·Published in Developments in biologicals·2012·Department Veterinary Medicines, Germany·View original on PubMed →
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Original publication title: Towards in vitro potency testing of inactivated erysipelas vaccines.
- Species:
- rodent
Plain-English summary
This research discusses how current methods for testing the effectiveness of inactivated swine erysipelas vaccines (vaccines for pigs) rely on using mice, which is becoming less favored due to advancements in technology and a push for more humane testing methods. The study suggests that analyzing the protein and immune responses of these vaccines in the lab could provide a reliable alternative that doesn't involve animals. The researchers found that vaccines with aluminum additives showed consistent results in their tests. However, they noted that the immune response from mice was quite different from that of pigs, which may affect how the vaccines are evaluated. Overall, the study points towards a promising new method for testing these vaccines without using animals.
Abstract
Ph. Eur. Monograph 0064 "Swine erysipelas vaccine (inactivated)" currently advises mouse serology for batch potency testing. However, technological advances in vaccine production, improved quality control systems and comprehensive post marketing surveillance increasingly promote the acceptance of non-animal approaches for batch release testing. Protein and immune profiles of inactivated swine erysipelas vaccines obtained by SDS-PAGE and Western Blot might offer a convenient global and functional in vitro alternative. Characteristic and consistent protein and immune profiles could be obtained for aluminium-adjuvanted vaccines. Immunoreactivity of polyclonal sera raised in mice differs markedly from reactivity of swine sera.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/22888593/