Peer-reviewed veterinary case report
TR-FRET Assays for Endogenous Huntingtin Protein Level in Mouse Cells.
- Journal:
- Journal of Huntington's disease
- Year:
- 2014
- Authors:
- Liang, Yijian et al.
- Affiliation:
- School of Life Sciences · China
Abstract
High-throughput measurement of huntingtin (Htt) levels is useful for Huntington's disease research. For example, identification of genetic or chemical modifiers that reduce Htt levels by high-throughput screening provides promising strategy for HD drug discovery. In the human cells, high-throughput measurement of Htt levels has been established based on the Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) technology, using the 2B7/MW1 antibody pair. Unfortunately, application of this assay in the mouse cells has been problematic due to discrepancies between TR-FRET signals and Western-blots, possibly caused by non-specific antibody binding. Here we report TR-FRET assays that are able to detect endogenous Htt levels of the mouse striatal cell line (STHdh).
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25300329/