Peer-reviewed veterinary case report
Tracking the growth fate of single cells and isolating slow-growing cells in human colorectal cancer organoids.
- Year:
- 2023
- Authors:
- Coppo R et al.
- Affiliation:
- Department of Clinical Bio-resource Research and Development · Japan
Abstract
Patient-derived tumor organoids are three-dimensionally cultured cancer cells that enable a suitable platform for studying heterogeneity and plasticity of cancer. We present a protocol for tracking the growth fate of single cells and isolating slow-growing cells in human colorectal cancer organoids. We describe steps for organoid preparation and culturing using the cancer-tissue-originating spheroid method, maintaining cell-cell contact throughout. We then detail a single-cell-derived spheroid-forming and growth assay, confirming single-cell plating, monitoring growth over time, and isolating slow-growing cells. For complete details on the use and execution of this protocol, please refer to Coppo et al.<sup>1</sup>.
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Search related cases →Original publication: https://europepmc.org/article/MED/37384521