Transcriptome comparative analysis revealed poly(I:C) activated RIG-I/MDA5-mediated signaling pathway in miiuy croaker.

Abstract

Miiuy croker (Miichthys miiuy) as an important economical aquaculture species has challenged many more diseases caused by various pathogens recently. To better explore the immune response to virus, we have analyzed the transcriptome profiling of miiuy croaker challenged with poly(I:C) synthetic analog of virus dsRNA. We have obtained differentially expressed genes (DEGs) with up/down-relevant from comparison of the Ctrl and Poly transcriptome libraries. Through GO and KEGG enrichment analysis, immune-relevant DEGs whose expression are significantly rise or fall after challenged have been identified and classified. In order to detailedly analysis host immune response patterns for dsRNA virus, we have performed a map based on RIG-I/MDA5-mediated and TLR3-mediated signaling pathway which both induced type I IFNs response. In this pathway, both MDA5 and LGP2 are important RLRs in host surveillance against infection of dsRNA viruses and induce type I IFNs response which subsequently form a transcription factor complex ISGF3 that promote downstream genes referred to as ISGs to inhibits virus replication.