Transcriptomic Analysis Reveals Functional Interaction of mRNA-lncRNA-miRNA inInfected by.

Abstract

The skin ofis a crucial component of the mucosal immune system and serves as the primary site of infection by. In order to investigate the significant role of skin ininfection, a comprehensive transcriptome analysis was conducted on skin tissues from the infection group, infection-adjacent group, and infection group compared with the infection-adjacent group (ATT_vs_PER, ADJ_vs_PER, ATT_vs_ADJ). This study identified differentially expressed long non-coding RNAs (DE lncRNAs), microRNAs (DE miRNAs), and differentially expressed genes (DEGs). The prediction of lncRNA target genes was accomplished by utilizing positional relationship (co-location) and expression correlation (co-expression) with protein-coding genes. Subsequently, functional enrichment analysis was conducted on the target genes of differentially expressed lncRNAs, revealing their involvement in signaling pathways such as tight junction, MAPK, and cell adhesion molecules. This study describes the regulatory network of lncRNA-miRNA-mRNA inskin tissue infected with. Functional prediction analysis showed that differentially expressed lncRNA and miRNA may regulate the expression of immune genes such as interleukin-8 () to resist the infection of. Conducting additional research on these non-coding RNAs will facilitate a deeper understanding of their immune regulatory function induringinfection. The study of non-coding RNA in this study laid a foundation for revealing the molecular mechanism of the immune system ofto respond to the infection of. It provided a choice for the molecular breeding ofagainst.