Peer-reviewed veterinary case report
Translation of Double‐Stranded RNA Segments 1 and 2 of Chuzan Virus in Rabbit Reticulocyte Lysates
- Journal:
- Journal of Veterinary Medicine, Series B
- Year:
- 1991
- Authors:
- Yoshida, Tatsumi et al.
- Species:
- rabbit
Abstract
SummaryCoding assignments of genome segments 1 and 2 of Chuzan virus strain K‐47 were studiedin vitroby using rabbit reticulocyte lysates. The double‐stranded RNA segment was extracted from sodium dodecyl sulfate (SDS)‐polyacrylamide gels by a modified crushing and eluting technique. Translation products labeled with [35S]methionine were resolved by SDS‐polyacrylamide gel electrophoresis. The molecular weights of the products from RNA segments 1 and 2 were estimated to be 98 and 95 kilodaltons, respectively.
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Search related cases →Original publication: https://doi.org/10.1111/j.1439-0450.1991.tb00920.x