Peer-reviewed veterinary case report
Cell sheet transplant from tunica vaginalis stops dog post-surgery
By Asano, Touko et al.·Published in Tissue engineering·2006·Department of Urology, Japan·View original on PubMed →
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Original publication title: Transplantation of an autologous mesothelial cell sheet prepared from tunica vaginalis prevents post-operative adhesions in a canine model.
- Species:
- dog
Plain-English summary
A group of male beagles underwent surgery that often leads to painful post-operative adhesions (scar tissue that can cause complications). After the surgery, some dogs received a special treatment using a sheet made from their own mesothelial cells, while others received a standard treatment or no treatment at all. Four weeks later, the dogs that received the mesothelial cell sheet had significantly fewer adhesions compared to the other groups. This suggests that using their own cells can help prevent painful scar tissue after surgery, potentially improving recovery for dogs undergoing similar procedures.
People also search for: dog surgery recovery · preventing adhesions in dogs · beagle post-operative care
Abstract
Post-operative adhesions often cause severe complications such as bowel obstruction and abdominopelvic pain. Previously, we reported that transplantation of a mesothelial cell sheet is effective for preventing adhesion in rat model. We also proposed a new technique for harvesting autologous mesothelial cells from tunica vaginalis without intra-abdominal maneuvers. In this study, we examined whether an autologous mesothelial cell sheet can prevent post-operative peritoneal adhesions in a canine adhesion model. Mesothelial cells were isolated from the tunica vaginalis of male beagles. Isolated cells were cultured on fibrin gel. We named this construct the "mesothelial cell sheet." Animals underwent surgery to induce peritoneal adhesion formation and were then transplanted with the mesothelial cell sheets (sheet group, n = 4), fibrin gel (fibrin group, n = 4), or no materials (sham group, n = 4). Four weeks after the transplantation, we evaluated adhesion formation and scored adhesion levels. The abdominal wall transplanted with the mesothelial cell sheet was covered with mesothelium. The total adhesion score of the sheet group was significantly lower than that of the fibrin group and the sham group. These results indicated that transplantation of an autologous mesothelial cell sheet is effective for preventing post-operative adhesion formation in the canine adhesion model. Our mesothelial cell sheet has the potential to be a powerful adhesion prophylactic material in surgery.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/16995796/