Peer-reviewed veterinary case report
Tris-EDTA boosts antibiotic effect on resistant dog ear infections
By Buckley, Laura M et al.·Published in Veterinary dermatology·2013·School of Veterinary Science, United Kingdom·View original on PubMed →
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Original publication title: Tris-EDTA significantly enhances antibiotic efficacy against multidrug-resistant Pseudomonas aeruginosa in vitro.
- Species:
- dog
Plain-English summary
A dog with chronic ear infections caused by a tough-to-treat bacteria called Pseudomonas aeruginosa was studied to see if adding a substance called Tris-EDTA could help antibiotics work better. The research found that when Tris-EDTA was combined with the antibiotics marbofloxacin and gentamicin, it significantly lowered the amounts needed to kill the bacteria. This means that using Tris-EDTA might help veterinarians treat stubborn ear infections in dogs more effectively.
People also search for: dog ear infection treatment · Pseudomonas aeruginosa in dogs · Tris-EDTA for dog antibiotics
Abstract
BACKGROUND: Multidrug-resistant Pseudomonas aeruginosa commonly complicates chronic bacterial otitis in dogs. HYPOTHESIS/OBJECTIVES: The aim of this in vitro study was to determine the effect of ethylenediaminetetraacetic acid-tromethamine (Tris-EDTA) on the minimal bactericidal concentrations (MBCs) and minimal inhibitory concentrations (MICs) of marbofloxacin and gentamicin for multidrug-resistant P. aeruginosa isolates from cases of canine otitis. METHODS: Eleven isolates were identified as multidrug resistant on disc diffusion; 10 were resistant to marbofloxacin and two were resistant to gentamicin. Isolates were incubated for 90 min with each antibiotic alone and in combination with Tris-EDTA at concentrations of 0.075 μg/mL to 5 mg/mL for marbofloxacin, 0.001 μg/mL to 10 mg/mL for gentamicin and 17.8:4.7 to 0.14:0.04 mg/mL for Tris-EDTA. Positive and negative controls were included. Aliquots of each antibiotic and/or Tris-EDTA concentration were subsequently transferred to sheep blood agar to determine the MBCs, and tryptone soy broth was added to the remaining suspensions to determine the MICs. RESULTS: Tris-EDTA alone was bacteriostatic but not bactericidal at any concentration. The addition of Tris-EDTA significantly reduced the median MBC (from 625 to 468.8 μg/mL; P < 0.001) and MIC (from 29.3 to 2.4 μg/mL; P = 0.008) of marbofloxacin, and the median MBC (from 625 to 39.1 μg/mL) and MIC (from 19.5 to 1.2 μg/mL) of gentamicin (both P < 0.001). CONCLUSIONS AND CLINICAL IMPORTANCE: Tris-EDTA significantly reduced the MBCs and MICs of marbofloxacin and gentamicin for multidrug-resistant P. aeruginosa in vitro. This may be of use to clinicians managing these infections in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/24025021/