Peer-reviewed veterinary case report
Trypanosoma vivax: a novel method for purification from experimentally infected sheep blood.
- Journal:
- Experimental parasitology
- Year:
- 2005
- Authors:
- González, L E et al.
- Affiliation:
- Universidad Simó
Abstract
Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and immunosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE-cellulose chromatography, with an estimated yield of 11-15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/16023641/