Two melatonin treatments improve the conception rate after fixed-time artificial insemination in beef heifers following synchronisation of oestrous cycles using the CoSynch-56 protocol.

Abstract

OBJECTIVE: This study investigated the effect of melatonin administration in combination with the CoSynch-56 protocol on the conception rate after artificial insemination (AI) in beef heifers. METHODS: Eighty-six beef heifers were allocated to four treatments in combination with CoSynch-56 treatment. All heifers, excluding the control group (CTR = 25), were injected with melatonin on day 1. The melatonin (MT = 20) and MT + human chorionic gonadotropin (MT + hCG = 21) groups received no further treatment with melatonin. Each heifer was treated with gonadotropin-releasing hormone (GnRH) on day 4, prostaglandin F2α (PGF2α) on day 11, and GnRH and AI 56 h later. The fourth group (2MT = 20) was injected again with melatonin concurrent with AI, and the MT + hCG group received hCG on day 19. Pregnancy diagnosis was performed using transrectal ultrasonography 45 days after AI and blood samples were collected via caudal venipuncture on days 0-1, 14-15, 24 and 59. Concentrations of progesterone (P4) and the activities of oxidative stress-related enzymes were determined using enzyme-linked immunosorbent assay. RESULTS: The conception rate was greater in the 2MT group (75%) than in the other groups, while there was no significant difference among the CTR (44%), MT (45%) and MT + hCG (38.1%) groups. Treatment with melatonin increased superoxide dismutase and glutathione peroxidase activities and decreased malondialdehyde concentrations but did not significantly affect the concentration of P4. CONCLUSION: Our results indicate that the administration of melatonin twice during the CoSynch-56 protocol may increase conception rates in beef heifers.