Typha pollen extract inhibit melanogenesis via α-MSH/MC1R signaling pathway in B16 and melasma mouse model.

Abstract

The aim of this study was to investigate the effects of Typha Pollen extract on melanogenesis in B16 and melasma mouse model. The effects of TP extract on B16 viability, melanin content of B16, MC1R content and TYR activity were studied by MTT assay, NaOH method, ELISA assay and dopa oxidation assay, respectively. In melasma mouse model, the effects of TP extract on MC1R content, MITF content and TYR activity were studied by ELISA assay, immunofluorescence staining and dopa oxidation assay, respectively. The results showed that the appropriate concentration of TP extract does not affect cell viability, and that TP extract inhibit B16 melanin, MC1R content and TYR activity. The results showed that TP extract inhibit skin MC1R content, MITF content and TYR activity. TP extract inhibit B16 melanin, inhibit proteins of α-MSH/MC1R signaling pathway such as MC1R, MITF and TYR. So TP extract is a natural and effective potential whitening agent.