Ultrastructural morphology of second and third-stage larvae ofinside paratenic host tissue.

Abstract

BACKGROUND: is a known cause of a zoonotic infectious illness called toxocariasis. Parathenic hosts are important as they can transmit larvae 2 (L) through direct transmission. Scanning electron microscope (SEM) techniques are needed to provide a three-dimensional image of each stage oflarvae. AIM: The aim of this study was to determine the morphology of Land Lin parathenic host tissue for etiological diagnosis using SEM. METHODS: Mice were used as suitable paratenic hosts for this experiment. A total of 786 embryonated eggs (16 eggs/gram body weight) containing Lwere inoculated into pregnant mice at day 11-13 of its gestation period. After delivery, Lwas transmitted to the off-spring. After 14 days, Lwas collected from mice, and Lwas collected from its off-spring. Data were analyzed descriptively based on ultrastructure examination using SEM. RESULTS: SEM examination results indicate that the size of Lis smaller than L. Results also showed differences between Land Lbased on middle and posterior observations. In the middle of the larval body, the number of Lbody rings was observed to be narrower and more than that of L. In addition, the distance between Lbody rings was much larger than that of L. Posteriorly, the tail tip of Lwas more curved than L. CONCLUSION: Ultrastructural examination using SEM has the ability to show differences in Land Lbody rings ofby observing the middle and posterior parts of its larvae.