Peer-reviewed veterinary case report
Uracil-DNA glycosylase-treated reverse transcription loop-mediated isothermal amplification for rapid detection of avian influenza virus preventing carry-over contamination.
- Journal:
- Journal of veterinary science
- Year:
- 2016
- Authors:
- Kim, Eun-Mi et al.
- Affiliation:
- College of Veterinary Medicine & Animal Disease Intervention Center · South Korea
- Species:
- bird
Abstract
Here, we describe a uracil-DNA glycosylase (UNG)-treated reverse transcription loop-mediated isothermal amplification (uRT-LAMP) for the visual detection of all subtypes of avian influenza A virus (AIV). The uRT-LAMP assay can prevent unwanted amplification by carryover contamination of the previously amplified DNA, although the detection limit of the uRT-LAMP assay is 10-fold lower than that of the RT-LAMP without a UNG treatment. To the best of our knowledge, this is the first successful application of deoxyuridine triphosphate/UNG strategy in RT-LAMP for AIV detection, and the assay can be applied for the rapid, and reliable diagnosis of AIVs, even in contaminated samples.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26726027/