Peer-reviewed veterinary case report
Early tibia fracture repair in dogs using bone matrix and TGF-beta1
By Servin-Trujillo, Miguel Angel et al.·Published in The Journal of veterinary medical science·2011·National Autonomous University of Mexico·View original on PubMed →
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Original publication title: Use of a graft of demineralized bone matrix along with TGF-β1 leads to an early bone repair in dogs.
- Species:
- dog
Plain-English summary
A group of young male dogs with tibia fractures underwent treatment using a special bone graft combined with a growth factor called TGF-β1 to help speed up healing. After five weeks, the dogs treated with this combination showed significant improvement in bone structure and early signs of healing, including the formation of new bone tissue. The treatment increased important proteins that help with bone repair, indicating that it was effective. Overall, using the graft with TGF-β1 helped the dogs heal faster than those who did not receive this treatment.
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Abstract
Tibia fractures are common in small animal practice. Over the past decade, improvements to animal internal fracture fixation have been developed. TGF-β1 has been shown to be crucial in the development, induction and repair of bone. In present study, we investigate the effect of local application of a graft demineralized bone matrix (DBM) along with TGF-β1 in a model of open osteotomy induced experimentally in dogs. Tibia fracture was brought about by using an open osteotomy model in young male dogs. Fracture repair was evaluated by a histological and biochemical analysis. Collagen content, proteolytic activity and urokinase-type plasminogen activator (uPA) expression were analyzed at the end of the study. Radiographic analysis, alkaline phosphatase and hematological evaluation were performed weekly. At the fifth week, there was an improvement and restoration of bone architecture in animals treated with a graft containing TGF-β1 (5 ng/ml) compared with the control and graft groups, as was evidenced by the presence of an early formation of wide callus and bone regeneration. In addition, local application of TGF-β1 led to an increase in collagen and proteolytic activity. More immunopositive osteoclast and mesenchymal cells were found in bone tissue from animals treated with TGF-β1 as compared with the control group. No changes in alkaline phosphatase, hematological and clinical parameters were observed. This study shows that the combined use of DBM along with TGF-β1 is able to improve and accelerate the bone repair.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/21566397/