Peer-reviewed veterinary case report
Detecting antibodies in dogs with chronic gut disease using a human
By Florey, J et al.·Published in Journal of veterinary internal medicine·2017·Department of Veterinary Clinical Sciences and Services, United Kingdom·View original on PubMed →
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Original publication title: Use of a Granulocyte Immunofluorescence Assay Designed for Humans for Detection of Antineutrophil Cytoplasmic Antibodies in Dogs with Chronic Enteropathies.
- Species:
- dog
Plain-English summary
A study looked at a new blood test for dogs that might help identify those with chronic gut issues, specifically food-responsive disease (FRD). Researchers found that this test, designed for humans, could effectively detect certain antibodies in dogs, making it easier and quicker than previous methods. The test showed a strong link between positive results and the diagnosis of FRD, which could help vets better understand and treat affected dogs. Overall, the new assay could be a useful tool for diagnosing chronic enteropathies in dogs.
People also search for: dog chronic diarrhea test · food-responsive disease in dogs · dog gut health blood test
Abstract
BACKGROUND: Perinuclear antineutrophil cytoplasmic antibodies (pANCA) previously have been shown to be serum markers in dogs with chronic enteropathies, with dogs that have food-responsive disease (FRD) having higher frequencies of seropositivity than dogs with steroid-responsive disease (SRD). The indirect immunofluorescence (IIF) assay used in previous publications is time-consuming to perform, with low interobserver agreement. HYPOTHESIS/OBJECTIVES: We hypothesized that a commercially available granulocyte IIF assay designed for humans could be used to detect perinuclear antineutrophil cytoplasmic antibodies in dogs. ANIMALS: Forty-four dogs with FRD, 20 dogs with SRD, 20 control dogs, and 38 soft-coated wheaten terrier (SCWT) or SCWT-cross dogs. METHODS: A granulocyte assay designed for humans was used to detect pANCA, cANCA, and antinuclear antibodies (ANA), as well as antibodies against proteinase-3 protein (PR-3) and myeloperoxidase protein (MPO) in archived serum samples. RESULTS: Sensitivity of the granulocyte assay to predict FRD in dogs was 0.61 (95% confidence interval (CI), 0.45, 0.75), and specificity was 1.00 (95% CI, 0.91, 1.00). A significant association was identified between positive pANCA or cANCA result and diagnosis of FRD (P < 0.0001). Agreement between the two assays to detect ANCA in the same serum samples from SCWT with protein-losing enteropathy/protein-losing nephropathy (PLE/PLN) was substantial (kappa, 0.77; 95% CI, 0.53, 1.00). Eight ANCA-positive cases were positive for MPO or PR-3 antibodies. CONCLUSIONS AND CLINICAL IMPORTANCE: The granulocyte immunofluorescence assay used in our pilot study was easy and quick to perform. Agreement with the previously published method was good.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/28608577/