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Peer-reviewed veterinary case report

Use of a surrogate chimeric virus to detect West Nile virus-neutralizing antibodies in avian and equine sera.

Journal:
Clinical and vaccine immunology : CVI
Year:
2009
Authors:
Komar, Nicholas et al.
Affiliation:
CDC-NCZVED-DVBID-ADB · United States

Abstract

A chimeric yellow fever virus/West Nile virus (WNV) was compared to WNV alone as a biosafety level 2 reagent in the plaque reduction neutralization test for determining WNV infection histories. Concordance was 96.3% among 188 avian and equine serum samples. Neutralizing antibody titers were frequently more than twofold lower with the chimera.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/19005021/