Use of biometric, hematologic, and plasma biochemical variables, and histopathology to assess the chronic effects of the herbicide prometryn on Common Carp.

Abstract

BACKGROUND: Effects of acute and subchronic exposure of fish to s-triazine herbicides have been well documented, but data on the effects of prometryn on blood analytes in carp at environmentally realistic concentrations are lacking. OBJECTIVE: The objective of the study was to determine whether biometrics, blood analytes, and histopathology could serve as bioindicators in fish living in waters polluted by triazine. METHODS: Fish were exposed to prometryn at concentrations of 0.51 (reported concentration in Czech rivers), 8.0, and 80 &#x3bc;g/L for 14, 30, and 60 days. Prior to and during this period, biometrics, blood analytes, and histopathology were evaluated. RESULTS: After 60 days' exposure to 8.0 and 80 &#x3bc;g/L of prometryn, spleen weights (P < .05) and lactate (P < .01) levels were significantly lower, and concentrations of HGB (P < .01) and MHC (P < .01) and MCHC (P < .01) were higher, relative to controls. After 30 and 60 days' exposure to 0.51, 8.0, and 80 &#x3bc;g/L of prometryn, AST activity, calcium, magnesium, and inorganic phosphate levels were lower (P < .01), while creatinine concentration and ALT activity were higher (P < .01) than in controls. Glucose was higher after exposure to 80 &#x3bc;g/L after 30 and 60 days, and after 60 days' exposure to 8.0 &#x3bc;g/L. Renal histology revealed severe hyaline degeneration of the epithelial cells of caudal kidney tubules in fish at all exposure levels. CONCLUSIONS: This study shows effects in fish blood at a concentration of 0.51 &#x3bc;g/L of prometryn, a significant finding in view of prometryn reaching a maximum of 4.4 &#x3bc;g/L or less in European rivers. Select blood analytes, such as creatinine, and histologic changes in caudal kidney are potential biomarkers for monitoring residual triazine pesticides in Common Carp.