Peer-reviewed veterinary case report
Using D-Squame tape to test skin immune markers in dogs with atopic
By Mosca, Marion et al.·Published in Veterinary Sciences·2024·Interactions Cells Environment, 2016. A104, VetAgro Sup, Dermatology Department, 69280 Marcy L’étoile, France, France·View original on Crossref →
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Original publication title: Use of D-Squame® as a Minimally Invasive Technique to Evaluate Skin Immune Response Biomarkers in Canine Atopic Dermatitis
- Species:
- dog
Plain-English summary
Eight beagle dogs with atopic dermatitis (a skin allergy) were studied to see how their skin reacted to allergens without needing painful biopsies. Researchers used a special tape called D-Squame® to collect skin samples and measure levels of certain immune markers. They found that levels of specific cytokines, which are proteins involved in inflammation, were significantly higher in affected areas of the skin. This suggests that D-Squame® could be a helpful tool for understanding skin allergies in dogs and identifying potential biomarkers for treatment.
People also search for: dog skin allergy treatment · beagle atopic dermatitis symptoms · D-Squame for dogs skin issues
Abstract
Evaluation of skin inflammation biomarkers in canine atopic dermatitis (AD) currently requires skin biopsies. Tape stripping has been shown to be a reliable technique to study biomarkers in the stratum corneum (SC) in humans. The aim of this study was to assess the immune response and identify biomarkers in the SC of dogs with canine AD using D-squame® as a minimally invasive technique. Eight beagle dogs were epicutaneously sensitized to Dermatophagoides farinae extract after tape stripping on sensitized site (S); twice a week for 49 days. Two sites were determined: lesional site (L) and non-lesional site (NL) on eight dogs affected spontaneously with AD. Adhesive tape strips D-Squame® were applied on each site. Skin concentrations of 10 cytokines were analyzed with an ELISA kit. Our results revealed a significant increase of IL-13, IL-4, and TNF-α concentrations in S and L sites. Regarding IFN-γ, its concentration was significantly increased in L skin and increased but not significantly in S sites. All the alarmins were not differentially expressed except IL-33 in the S site. IL-31, IL-1β, and IL-10 were not detectable. D-squame® seems to be a suitable technique to extract inflammatory cytokines from the SC of dogs, and IL-13, IL-4, TNF-α, and IFN-γ could be interesting biomarkers of canine AD.
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Search related cases →Original publication on Crossref: https://doi.org/10.3390/vetsci12010004