Peer-reviewed veterinary case report
Use of FTA card for the detection of two RNA (CSFV and SV-A) and two DNA viruses (ASFVand SuHV-1) of importance in veterinary medicine.
- Journal:
- Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
- Year:
- 2024
- Authors:
- Fonseca, Antônio Augusto et al.
- Affiliation:
- Laborató
Plain-English summary
Researchers have been looking into a new tool called the FTA card, which is a special type of filter paper that helps preserve and stabilize genetic material from samples. This study focused on using the FTA card to detect four important viruses that can affect pigs: two RNA viruses, Senecavirus A and classical swine fever virus, and two DNA viruses, African swine fever virus and suid alphaherpesvirus 1. While the FTA card showed good reliability and was easy to use for transporting samples, it did have some limitations, as it was less sensitive in detecting these viruses compared to other methods. Overall, the FTA card could be a useful option for handling samples, but it may not always catch every instance of these viruses.
Abstract
The FTA card has emerged as a promising alternative for nucleic acid extraction. The FTA card is a filter paper impregnated with chemicals that preserve and stabilize the genetic material present in the sample, allowing for its storage and transport at room temperature. The aim of this study was to test the card for the detection of RNA and DNA nucleic acids. Two RNA viruses (Senecavirus A and classical swine fever virus) and two DNA viruses (African swine fever virus and suid alphaherpesvirus 1) were tested, and in all cases, there was a decrease in sensitivity. The methods exhibited good repeatability and demonstrated a rapid and practical use for sample transport and nucleic acid extraction.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/38589741/