Peer-reviewed veterinary case report
Improving cat body fluid cell counts with hyaluronidase and ADVIA 120
By Lee, Sei Ming et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2017·School of Veterinary Medicine (Lee), United Kingdom·View original on PubMed →
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Original publication title: Use of hyaluronidase in the comparison between manual and automated hematology analysis with the ADVIA 120 to improve analysis of feline body cavity effusions.
- Species:
- cat
Plain-English summary
A cat with fluid buildup in its abdomen had trouble getting an accurate white blood cell count due to clumping of cells in the sample. Researchers found that treating the fluid sample with hyaluronidase, an enzyme that breaks down thick substances, helped improve the accuracy of the cell count. After this treatment, the results from an automated analyzer matched better with manual counting methods, especially in samples where the cells were clumped together. This suggests that using hyaluronidase before testing can lead to more reliable results for diagnosing issues related to fluid in cats.
People also search for: cat fluid buildup treatment · feline effusion diagnosis · hyaluronidase for cats
Abstract
Classification of body cavity effusions is an important step in the investigation and diagnosis of disease in cats. Feline inflammatory effusions are often highly proteinaceous and viscous, which can cause clumping of white cells and subsequently inaccurate nucleated cell counts (NCCs) using automated and manual methods. Microscopic assessment of cellularity can also be difficult given the variable thickness of smears and cell clumping, which skews white cell distribution. The ADVIA 120 uses 2 white cell-counting channels, the basophil/lobularity (WBC/baso) and differential/peroxidase channels, which can provide quite different results in highly viscous feline samples and often disagree with smear assessment of cellularity. We investigated the effects of pre-incubation of feline effusion samples with hyaluronidase and its effects on NCCs and cellularity assessment. NCCs were obtained by automated analysis using the ADVIA 120 and by manual counting methods. Agreement was assessed using a Bland-Altman chart. Pretreatment of samples with hyaluronidase resulted in good agreement between the ADVIA basophil channel and manual counting methods in all samples in the study. However, improvements in NCCs after hyaluronidase treatment were significantly greater in clumped samples, and cell distribution of these samples on direct smears was also improved. Therefore, when nucleated cell clumping is observed on a direct smear, pretreatment of the sample with hyaluronidase prior to analysis on an automated analyzer is advised, with the WBC/baso channel displaying the most accurate NCC.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/28064834/