Peer-reviewed veterinary case report
Use of reverse transcriptase PCR to subtype N1 to N9 neuraminidase genes of avian influenza viruses.
- Journal:
- Journal of clinical microbiology
- Year:
- 2009
- Authors:
- Tsukamoto, Kenji et al.
- Affiliation:
- National Institute of Animal Health · Japan
- Species:
- bird
Abstract
Reverse transcriptase PCR designed to amplify the N1 to N9 neuraminidase (NA) genes of avian influenza viruses detected 118 of the 119 NA genes tested (99.2%) in a subtype-specific manner. This technique successfully subtyped all 167 recent avian influenza viruses isolated from birds. Subtype specificity was confirmed by sequence analyses of all 285 PCR products.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/19403772/