Peer-reviewed veterinary case report
Using CF0218-ELISA to distinguish Chlamydophila felis-infected cats from vaccinated and uninfected domestic cats.
- Journal:
- Veterinary microbiology
- Year:
- 2010
- Authors:
- Ohya, Kenji et al.
- Affiliation:
- Faculty of Applied Biological Sciences · Japan
- Species:
- cat
Plain-English summary
Chlamydophila felis is a germ that can cause eye infections and pneumonia in cats. While there are vaccines available to protect cats from this germ, it can be hard to tell if a cat is infected or just vaccinated because current tests don’t differentiate between the two. Researchers tested a new blood test called CF0218-ELISA, which can identify antibodies specific to Chlamydophila felis, in a group of 714 cats with known vaccination histories. They found that 93% of vaccinated cats tested negative for the infection, while a smaller percentage of unvaccinated cats showed signs of infection. This new test appears to be effective in telling apart infected cats from those that have been vaccinated or are not infected at all.
Abstract
Chlamydophila felis is a causative agent of acute and chronic conjunctivitis and pneumonia in cats. Cats can be vaccinated with killed or attenuated C. felis. However, current serodiagnostics cannot distinguish these cats from naturally infected cats. This causes difficulty of early diagnosis and seroepidemiological survey for C. felis. We previously reported that C. felis CF0218 can be used as a C. felis-infection-specific diagnostic antigen in experimentally infected and/or vaccinated cats. In this study, we evaluated an enzyme-linked immunosorbent assay using recombinant CF0218 as antigen (CF0218-ELISA) to detect anti-C. felis antibody in 714 sera of domestic cats whose histories of vaccination against C. felis are known. The 44 vaccinated cats were 93% negative using CF0218-ELISA; half of these scored positive by immunofluorescence assay (IFA) using C. felis-infected cells as antigen. The 670 non-vaccinated cats had CF0218-ELISA positivity rates that were statistically in agreement with IFA (18% vs. 21%). These results show that CF0218, which was identified as a C. felis-infection-specific antigen, is a useful serodiagnostic antigen to distinguish naturally C. felis-infected cats from vaccinated and non-infected cats.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21095510/