Peer-reviewed veterinary case report
Using Drosophila larvae to study epidermal wound closure and inflammation.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2013
- Authors:
- Burra, Sirisha et al.
- Affiliation:
- Department of Biochemistry and Molecular Biology · United States
Abstract
This methods chapter describes two methods for creating epithelial wounds in Drosophila larvae: pinch and puncture wounding. It also covers protocols for visualizing epithelial wounds, either in a dissected whole mount preparation or, using transgenic reporter larvae, in a live whole mount preparation. Finally, useful transgenic lines for live genetic screening of genes required for wound closure or inflammation are described.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24029952/